Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 12, Pages 6153-6159Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac501375s
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Funding
- National Natural Science Foundation of China [21375073, 21235004, 21275082]
- National Basic Research Program of China [2011CB935704, 2013CB934004]
- European Union [260600]
- Tsinghua University Initiative Scientific Research Program
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A highly sensitive electrochemical biosensor was built for the detection of kinase activity based on the DNA induced gold nanoparticles (AuNPs) polymeric network block signal amplification. In this strategy, the DNA, conjugated AuNPs were integrated with the phosphorylated peptide by Zr4+ and assembled into DNA-AuNPs polymeric network block by the hybridization of cDNA with each side sequences of DNA, and joint DNA(2). The kinase activity was determined by the amperometric responses of [Ru(NH3)(6)](3+) absorbed on the network block by electrostatic interaction. Due to its excellent electroactivity and high accommodation of the DNA- AuNPs polymeric network block for [Ru(NH3)(6)](3+), the current signal was significantly amplified, affording a highly sensitive electrochemical analysis of kinase activity. The asproposed biosensor presents a low detection limit of 0.03 U mL(-1) for protein kinase A (PICA) activity, wide linear range (from 0.03 to 40 U mL(-1)), and excellent stability even in cell lysates and serum samples. This biosensor can also be applied for quantitative kinase inhibitor screening. Finally, the PICA activities from BE4S-2B, A549, and MCF-7 cell lysates were further analyzed, which provided a valuable strategy in developing a high-throughput assay of in vitro kinase activity and inhibitor screening for clinic diagnostics and therapeutics.
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