Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1154, Issue 1-2, Pages 373-378Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.02.029
Keywords
capillary electrophoresis; electrochemiluminescence; chloroquine phosphate; human serum albumin
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This paper describes a novel and sensitive method for the estimation of chloroquine phosphate (CU using capillary electrophoresis with end-column electrochemiluminescence (ECL) detection. Under the optimized condition, linear calibration curve was obtained for the system over two orders of magnitude with a detection limit of 3 x 10(-7) M (S/N=3). The relative standard deviations of the ECL intensity and the migration time were 1.4% and 0.05%, respectively (n=6, 5 x 10(-5) M CQ). Successful separation of chloroquine phosphate, difenidol hydrochloride and clomifene citrate was obtained at pH 7.0. Using the proposed electrochemiluminescence system, a simple method was proposed to study the interaction between chloroquine phosphate and human serum albumin (HSA), and the number of binding sites and binding constant were estimated to be 32.6 and 7.7 x 10(3) M-1, respectively. (c) 2007 Elsevier B.V All rights reserved.
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