Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 2, Pages 982-986Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac402823n
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Funding
- Czech Science Foundation [GCP206/10/J012, GAP206/12/0538]
- European Regional Development Fund [CZ.1.05/1.1.00/02.0068]
- program of Employment of Newly Graduated Doctors of Science for Scientific Excellence [CZ.1.07/2.3.00/30.009]
- state budget of the Czech Republic
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing highspeed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in similar to 11 min using 100 laser shots per pixel. This is almost an order Of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
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