Journal
JOURNAL OF NEUROSCIENCE
Volume 27, Issue 26, Pages 7028-7040Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1624-07.2007
Keywords
retinal stem cells; Muller glia; photoreceptors; regeneration; zebrafish; radial glia
Categories
Funding
- NEI NIH HHS [R01EY004318, R01 EY004318, F32 EY017251] Funding Source: Medline
- NINDS NIH HHS [F31 NS047909] Funding Source: Medline
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Neuronal progenitors in the mammalian brain derive from radial glia or specialized astrocytes. In developing neural retina, radial glia-like Muller cells are generated late in neurogenesis and are not considered to be neuronal progenitors, but they do proliferate after injury and can express neuronal markers, suggesting a latent neurogenic capacity. To examine the neurogenic capacity of retinal glial cells, we used lineage tracing in transgenic zebrafish with a glial-specific promoter (gfap, for glial fibrillary acid protein) driving green fluorescent protein in differentiated Mu r ller glia. We found that all Muller glia in the zebrafish retina express low levels of the multipotent progenitor marker Pax6 (paired box gene 6), and they proliferate at a low frequency in the intact, uninjured retina. Muller glia-derived progenitors express Crx (cone rod homeobox) and are late retinal progenitors that generate the rod photoreceptor lineage in the postembryonic retina. These Muller glia-derived progenitors also remain competent to produce earlier neuronal lineages, in that they respond to loss of cone photoreceptors by specifically regenerating the missing neurons. We conclude that zebrafish Muller glia function as multipotent retinal stem cells that generate retinal neurons by homeostatic and regenerative developmental mechanisms.
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