Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 358, Issue 2, Pages 424-428Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2007.04.154
Keywords
pattern; E. coli; recognition; glycolipid; HSA
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We demonstrated that human serum albumin (HSA) patterns constructed in a solid substrate by using micro-contact printing (mu CP) technique supported the deposition of phospholipid bilayer containing glycolipid, 10-tetradecyloxymethy-3,6,9,12-tetraoxahexacosyl 2-acetamido-2-deoxy-beta-D-glucopyranoside (PB1124). It is observed by confocal laser scanning microscopy (CLSM) that the obtained glycolipid patterns are well-defined, stable and can be used to recognize and immobilize Escherichia coli (E. coli). This strategy is promising to perform bacterial detection through solid surface recognition in a way of biosensors. (C) 2007 Elsevier Inc. All rights reserved.
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