Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 19, Pages 9126-9134Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4017715
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Funding
- National Center for Research Resources
- National Institute of General Medical Sciences of the National Institutes of Health through the National Flow Cytometry Resource [P41 RR01315]
- Laboratory Directed Research and Development award from Los Alamos National Laboratory [20130239ER]
- International Design Center from Singapore University of Technology and Design-Massachusetts Institute of Technology Alliance [IDG11300101]
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A microfluidic device was developed to separate heterogeneous particle or cell mixtures in a continuous flow using acoustophoresis. In this device, two identical surface acoustic waves (SAWs) generated by interdigital transducers (IDTs) propagated toward a microchannel, which accordingly built up a standing surface acoustic wave (SSAW) field across the channel. A numerical model, coupling a piezoelectric effect in the solid substrate and acoustic pressure in the fluid, was developed to provide a better understanding of SSAW-based particle manipulation. It was found that the pressure nodes across the channel were individual planes perpendicular to the solid substrate. In the separation experiments, two side sheath flows hydrodynamically focused the injected particle or cell mixtures into a very narrow stream along the centerline. Particles flowing through the SSAW field experienced an acoustic radiation force that highly depends on the particle properties. As a result, dissimilar particles or cells were laterally attracted toward the pressure nodes at different magnitudes, and were eventually switched to different outlets. Two types of fluorescent microspheres with different sizes were successfully separated using the developed device. In addition, Escherichia coli bacteria premixed in peripheral blood mononuclear cells (PBMCs) were also efficiently isolated using the SSAW-base separation technique. Flow cytometric analysis on the collected samples found that the purity of separated E. coli bacteria was 95.65%.
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