Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 12, Pages 5632-5636Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac401056q
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Funding
- National Science Foundation [CHE 0847205, CHE 0848650]
- National Science Foundation Instrumentation Development for Biological Research [DBI 0852740]
- National Center for Research Resources [5R51RR031246-02]
- National Institute of General Medical Sciences from the National Institutes of Health [8R21GM103454]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852740] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [847205] Funding Source: National Science Foundation
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We describe a sampling method using glass capillaries for quantitative analysis of trace analytes in small volumes of complex mixtures (similar to 1 mu L) using ambient ionization mass spectrometry. The internal surface of a sampling glass capillary was coated with internal standard then used to draw liquid sample and so transfer both the analyte and internal standard in a single fixed volume onto a substrate for analysis. The internal standard was automatically mixed into the sample during this process and the volumes of the internal standard solution and sample are both fixed by the capillary volume. Precision in quantitation is insensitive to variations in length of the capillary, making the preparation of the sampling capillary simple and providing a robust sampling protocol. Significant improvements in quantitation accuracy were obtained for analysis of 1 mu L samples using various ambient ionization methods.
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