Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 14, Pages 6915-6921Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac401334r
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Funding
- National Basic Research Program 973 [2011CB933600, 2010CB732600]
- Award for the Hundred Talent Program of the Chinese Academy of Science
- Natural Science Foundation of China [21075129, 21205128]
- Fund for Shenzhen Engineering Laboratory of Single-Molecule Detection and Instrument Development [433]
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We develop a new homogeneous method for sensitive detection of various biomolecules on the basis of bioluminescence monitoring the released AMP from the target-triggered hybridization chain reaction-mediated ligation. The introduction of hybridization chain reaction not only improves the sensitivity of DNA assay, but also facilitates the sensitive detection of proteins by designing specific aptamer triggers, providing a universally amplified platform for simultaneous detection of different kinds of biomolecules. Importantly, this bioluminescence assay employs the target-dependent ATP from the ligation byproduct of AMP as the reporter without the requirement for the sophisticated luciferase manipulation, complicated immobilization, and separation steps. The proposed method has significant advantages of simplicity, high sensitivity, low cost, and high throughput, and holds a great promise for practical point-of-care applications.
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