Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 24, Pages 11929-11935Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4027753
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Funding
- National Basic Research Program of China (973 Program) [2011CB933600]
- 863 program [2013AA032204]
- Science Fund for Creative Research Groups of NSFC [20921062]
- National Natural Science Foundation of China [81201193, 2117510]
- Program for New Century Excellent Talents in University [NCET-10-0656]
- 111 Project [111-2-10]
- 3551 Talent Program of the Administrative Committee of East Lake Hi-Tech Development Zone [[2011] 137]
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In this study, we report a simple method for simultaneous detection of multiplex DNA sequences, including complementary DNA (cDNA) sequences of HIV and HCV, DNA sequence of HBV, with QDs-encoded fluorescent nanospheres and nano-gamma-Fe2O3-coated magnetic nanospheres. Detection was achieved on a fluorescence spectrophotometer without additional auxiliary instruments, and the detection limit was about 100 pM. Here, QDs-encoded fluorescent nanospheres (FNS) with different photoluminescent properties, and magnetic nanospheres (MNS) were separately fabricated by stepwise assembly of hydrophobic QDs or nano-gamma-Fe2O3 on the surface of branched poly(ethylene imine) (PEI)-coated nanospheres in precisely controlled amounts, finally followed by silica encapsulation. FNS-labeled probe DNAs and MNS-labeled capture DNAs were used to hybridize with the corresponding targets at the same time. After magnetic separation, the sandwich-structured adducts were measured by fluorescence spectrophotometry. The results indicated that the targets could be detected with high sensitivity. This method is convenient, fast enough, and capable of high anti-interference. Therefore, it is expected to be used for simultaneous detection and separation of multiple targets at high levels of purity and throughput.
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