Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 16, Pages 7875-7881Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac401518e
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Funding
- National Key Scientific Program of China [2011CB911000]
- NSFC [J1210040, 20975034, 21177036, 21275044]
- Foundation for Innovative Research Groups of NSFC [21221003]
- National Key Natural Science Foundation of China [21135001]
- National Instrumentation Program [2011YQ030124]
- Ministry of Education of China [20100161110011]
- Hunan Provincial Natural Science Foundation [11111002]
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H2S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better understand its physiological and pathological functions, efficient methods for monitoring of H2S in living systems are desired. Although quite a few one photon fluorescence probes have been reported for H2S, two-photon (TP) probes are more favorable for intracellular imaging. In this work, by employing a donor-g-acceptor-structured naphthalene derivative as the two-photon fluorophore and an azide group as the recognition unit, we reported a new two-photon bioimaging probe 6(benzo[d]thiazol-2'-yl)-2-azidonaphthalene (NHS1) for H2S with improved sensitivity. The probe shows very low background fluorescence in the absence of H2S. In the presence of H2S, however, a significant enhancement for both one photon and TP excited fluorescence were observed, resulting in a high sensitivity to H2S in aqueous solutions with a detection limit of 20 nM observed, much lower than the previously reported TP probe. The probe also exhibits a wide linear response concentration range (0-5 mu M) to H2S with high selectivity. All these features are favorable for direct monitoring of H2S in complex biological samples. It was then applied for direct TP imaging of H2S in living cells with satisfactory sensitivity, demonstrating its value of practical application in biological systems.
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