Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 4, Pages 2356-2360Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac303356v
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Funding
- National Institute of Health [1R01AI096226-01]
- NHLBI NRSA [F30 HL110723]
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Time-lapse or longitudinal fluorescence microscopy is broadly used in cell biology. However, current available time-lapse fluorescence microscopy systems are bulky and costly. The limited field-of-view (FOV) associated with the microscope objective necessitates mechanical scanning if a larger FOV is required. Here we demonstrate a wide FOV time-lapse fluorescence self-imaging Petri dish system, termed the Talbot Fluorescence ePetri, which addresses these issues. This system's imaging is accomplished through the use of the Fluorescence Talbot Microscopy (FTM). By incorporating a microfluidic perfusion subsystem onto the platform, we can image cell cultures directly from within an incubator. Our prototype has a resolution limit of 1.2 mu m and an FOV of 13 mm(2). As demonstration, we obtained time-lapse images of He La cells expressing H2B-eGFP. We also employed the system to analyze the cells' dynamic response to an anticancer drug, camptothecin (CPT). This method can provide a compact and simple solution for automated fluorescence imaging of cell cultures in incubators.
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