Journal
PLANT SCIENCE
Volume 173, Issue 1, Pages 12-24Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2007.03.013
Keywords
adventitious root; Bupleurum kaoi; cDNA microarray; methyl jasmonate (MeJA); relative quantification in real-time PCR (qRT-PCR)
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The plant regulator methyl-jasmonate (MeJA) mediates diverse developmental processes and defense responses which induce a variety of secondary metabolites. This study reports the regulatory network of MeJA-induced genes in adventitious root cultures of the medicinal herb Bupleurum kaoi. A cDNA microarray containing 465 unique expressed sequence tags (ESTs) as probe sets, generated with a PCR-Select cDNA Subtraction Kit, was employed to profile the transcriptome of MeJA induction at thirteen points in time. Real-time PCR was performed to verify changes in expression of 36 unique ESTs (uniESTs), detected via microarray. Genes upregulated by McJA include those involved in saikosaponin, amino acid, phenylpropanoid and jasmonate biosynthesis, defense signaling, plant hormone regulation and stress protection; indicating that the JA signaling pathway interacts with other signaling pathways leading to transcriptional reprogramming in B. kaoi adventitious roots. Some of these MeJA-induced genes have applications in agricultural, pharmaceutical and food industries. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
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