Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 12, Pages 6080-6088Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac400949t
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Funding
- US Department of Energy
- Office of Science, Basic Energy Sciences, via DOE grant [DE-FG02-90ER14114, DE-FG02-05ER46249]
- US National Science Foundation via NSF-NIRT grant [CTS-0304055, CBET-1122780]
- National Institutes of Health via NIH grant [GM44844]
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In order to measure the intermolecular binding forces between two halves (or partners) of naturally split protein splicing elements called inteins, a novel thiol-hydrazide linker was designed and used to orient immobilized antibodies specific for each partner. Activation of the surfaces was achieved in one step, allowing direct intermolecular force measurement of the binding of the two partners of the split intein (called protein trans-splicing). Through this binding process, a whole functional intein is formed resulting in subsequent splicing. Atomic force microscopy (AFM) was used to directly measure the split intein partner binding at 1 mu m/s between native (wild-type) and mixed pairs of C- and N-terminal partners of naturally occurring split inteins from three cyanobacteria. Native and mixed pairs exhibit similar binding forces within the error of the measurement technique (similar to 52 pN). Bioinformatic sequence analysis and computational structural analysis discovered a zipper-like contact between the two partners with electrostatic and nonpolar attraction between multiple aligned ion pairs and hydrophobic residues. Also, we tested the Jarzynskis equality and demonstrated, as expected, that nonequilibrium dissipative measurements obtained here gave larger energies of interaction as compared with those for equilibrium. Hence, AFM coupled with our immobilization strategy and computational studies provides a useful analytical tool for the direct measurement of intermolecular association of split inteins and could be extended to any interacting protein pair.
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