Journal
SENSORS
Volume 7, Issue 7, Pages 1091-1107Publisher
MDPI
DOI: 10.3390/s7071091
Keywords
calix[4] arene; self-assembled monolayer; linker system; anti-hIgG; biosensor
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Three calix[4] arene (Cal- 4) derivatives of which contain ethylester (1), carboxylic acid ( 2), and crownether ( 3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers ( SAMs) on Au films. After spectroscopic characterization of monolayers, the interaction between Cal- 4 and surface confined bovine serum albumin (BSA) in the SAMs was analyzed by surface plasmon resonance (SPR). The estimated surface concentration of BSA on the Cal- 4 SAM with crownether group was the highest among the three Cal-4 derivatives. Anti- hIgG and hIgG pair was employed for the investigation of protein-protein interaction. Molecular interaction between anti- hIgG and hIgG can be detected in a concentration range of 10 pg/ mL to 200 pg/mL on the Cal- 4 derivative 3 SAM modified SPR chip.
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