Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 20, Pages 9721-9727Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac4021227
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Funding
- 973 Program [2013CB933800]
- National Natural Science Foundation of China [21035003, 21227005, 21205074]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20113704130001, 20123704120006]
- Program for Changjiang Scholars and Innovative Research Team in University
- Chinese Ministry of Education [212102]
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In this paper, we have developed a biofriendly and high sensitive apo-GO(x) (inactive form of glucose oxidase)-modified gold nanoprobe for quantitative analysis of glucose and imaging of glucose consumption in living cells. This detection system is based on fluorescence resonance energy transfer between apo-GO(x) modified AuNPs (Au nanoparticles) and dextran-FITC (dextran labeled with fluorescein isothiocyanate). Once glucose is present, quenched fluorescence of FITC recovers due to the higher affinity of apo-GO(x) for glucose over dextran. The nanoprobe shows excellent selectivity toward glucose over other monosaccharides and most biological species present in living cells. A detection limit as low as 5 nM demonstrates the high sensitivity of the nanoprobe. Introduction of apo-GO(x), instead of GO(x), can avoid the consumption of O-2 and production of H2O2 during the interaction with glucose, which may exert effects on normal physiological events in living cells and even lead to cellular damage. Due to the low toxicity of this detection system and reliable cellular uptake ability of AuNPs, imaging of intracellular glucose consumption was successfully realized in cancer cells.
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