Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 8, Pages 3832-3835Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac303569t
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Funding
- Japan Society for the Promotion of Science (JSPS) [22245011, 23760745]
- Cabinet Office, Government of Japan, through its Funding Program for Next Generation World-Leading Researchers
- Grants-in-Aid for Scientific Research [22245011, 24710140, 23760745] Funding Source: KAKEN
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In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.
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