Journal
ANALYTICAL CHEMISTRY
Volume 85, Issue 18, Pages 8498-8502Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac402214g
Keywords
-
Categories
Funding
- NSF [CHE-0847642, BIO-1062846]
- DOE [DE-FG02-98EF14890]
Ask authors/readers for more resources
Work presented here demonstrates application of nanopipettes pulled to orifice diameters of less than 100 nm as electrospray ionization emitters for mass spectrometry. Mass spectrometric analysis of a series of peptides and proteins electrosprayed from pulled-quartz capillary nanopipette emitters with internal diameters ranging from 37 to 70 nm is detailed. Overall, the use of nanopipette emitters causes a shift toward the production of ions of higher charge states and leads to a reduction in width of charge-state distribution as compared to typical nanospray conditions. Further, nanopipettes show improved S/N and the same signal precision as typical nanospray, despite the much smaller dimensions. As characterized by SEM images acquired before and after spray, nanopipettes are shown to be robust under conditions employed. Analytical calculations and numerical simulations are used to calculate the electric field at the emitter tip, which can be significant for the small diameter tips used.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available