Journal
ANALYTICAL CHEMISTRY
Volume 86, Issue 1, Pages 308-311Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac403226h
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Funding
- National Natural Science Foundation of China [21075094]
- National Basic Research of China (973 program) [2011CB933600]
- Program for New Century Excellent Talents in University [NCET-11-0402]
- NRF [NRF-2012R1A2A1A03670456]
- Korean Government
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A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO. Adopting a quinoline derivative as the fluorophore, QNO has a large two-photon action cross section value of 52 GM and long-wavelength emission. It also features high selectivity, low cytotoxicity, and pH insensitivity. By utilizing two-photon microscopy (TPM), QNO can detect NO in live cells and live tissues at a depth of 90-180 mu m.
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