Journal
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Volume 43, Issue 1, Pages 73-84Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2007.04.014
Keywords
adiponectin; AdipoR1; AdipoR2; rosiglitazone; AMPK; fatty acid oxidation; glucose uptake; cardiomyocytes
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Funding
- NCRR NIH HHS [C06 RR07571] Funding Source: Medline
- NHLBI NIH HHS [1R01HL084456, R01 HL084456-01A1, R01 HL085499-01, R01 HL084456, R01 HL085499, 1R01HL085499] Funding Source: Medline
- NIGMS NIH HHS [S06 GM008248-200058, S06GM08248, S06 GM008248] Funding Source: Medline
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Adiponectin is a protein hormone involved in maintaining energy homeostasis in metabolically active tissues. It enhances glucose and lipid metabolism via activation of AMP-dependent kinase (AMPK) in skeletal muscle and liver. Energy homeostasis is vital for the heart to work as a pump. In this study, we investigated whether adiponectin and its receptors are expressed in adult ventricular cardiomyocytes. We observed adiponectin transcript and protein in cultured ventricular cardiomyocytes isolated from adult rat, by quantitative real-time PCR, ELISA assays, Western blots, and immunofluorescent staining. In addition, we detected adiponectin receptor (AdipoR1 and AdipoR2) expression in the heart. AdipoR I was expressed in rat myocardium at a level of similar to 50% of that in skeletal muscle; whereas adipoR2 was expressed at a similar level to that in liver. Rosiglitazone, a Peroxisome proliferator activated receptor gamma (PPAR-gamma) activator, substantially elevated expression of adiponectin in cultured cardiomyocytes and its secretion into cultured media. Rosiglitazone also increased adipoR1 and adipoR2 expression in cardiomyocytes. Treatment of recombinant globular adiponectin in cultured cardiomyocytes increased fatty acid oxidation and glucose uptake via activation of AMPK, suggesting a role for adiponectin in cardiac energy metabolism. Together, these data establish the existence of a local cardiac-specific adiponectin system that is regulated by PPAR-gamma. Moreover, these findings indicate a role for adiponectin on normal myocardial energy homeostasis, in part, through the activation of AMPK. (C) 2007 Elsevier Inc. All rights reserved.
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