Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 22, Pages 9668-9673Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac3025366
Keywords
-
Categories
Funding
- Netherlands Proteomics Center
- PRIME-XS project [262067]
- European Union
- Netherlands Organization for Scientific Research (NWO) [700.10.429]
Ask authors/readers for more resources
Increasing peptide sequence coverage by tandem mass spectrometry improves confidence in database search-based peptide identification and facilitates mapping of post-translational modifications and de novo sequencing. Inducing 2-fold fragmentation by combining electron-transfer and higher-energy collision dissociation (EThcD) generates dual fragment ion series and facilitates extensive peptide backbone fragmentation. After an initial electron-transfer dissociation step, all ions including the unreacted precursor ions are subjected to collision induced dissociation which yields b/y- and c/z-type fragment ions in a single spectrum. This new fragmentation scheme provides richer spectra and substantially increases the peptide sequence coverage and confidence in peptide identification.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available