Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 18, Pages 7607-7612Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac302117z
Keywords
-
Categories
Funding
- NIH [GM074739, GM082848]
- Nanoscale Science and Engineering Initiative of the National Science Foundation [CHE-0641523]
- New York State Office of Science, Technology, and Academic Research (NYSTAR)
Ask authors/readers for more resources
Single-molecule imaging of biological macromolecules has dramatically impacted our understanding of many types of biochemical reactions. To facilitate these studies, we have established new strategies for anchoring and organizing DNA molecules on the surfaces of microfluidic sample chambers that are otherwise coated with fluid lipid bilayers. This previous work was reliant upon the use of double-stranded DNA, precluding access to information on biological processes involving single-stranded nucleic acid substrates. Here, we present procedures for aligning and visualizing single-stranded DNA molecules along the leading edges of nanofabricated barriers to lipid diffusion, in both single-tethered and double-tethered experimental formats. This new single-molecule approach provides long-awaited access to critical biological reactions involving single-stranded DNA binding proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available