Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 9, Pages 4153-4160Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac3004055
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Funding
- National Cancer Institute [U54CA151880]
- Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF [EEC-0647560]
- Prostate Cancer Foundation
- Howard Hughes Medical Institute
- Northwestern Memorial Foundation Dixon Translational Research Grant Initiative [SP0010333]
- King Abdullah Scholarships Program
- Ministry of Higher Education of Saudi Arabia
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We report the development of a novel Scanometric MicroRNA (Scano-miR) platform for the detection of relatively low abundance miRNAs with high specificity and reproducibility. The Scano-miR system was able to detect 1 fM concentrations of miRNA in serum with single nucleotide mismatch specificity. Indeed, it provides increased sensitivity for miRNA targets compared to molecular fluorophore-based detection systems, where 88% of the low abundance miRNA targets could not be detected under identical conditions. The application of the Scano-miR platform to high density array formats demonstrates its utility for high throughput and multiplexed miRNA profiling from various biological samples. To assess the accuracy of the Scano-miR system, we analyzed the miRNA profiles of samples from men with prostate cancer (Cap), the most common noncutaneous malignancy and the second leading cause of cancer death among American men. The platform exhibits 98.8% accuracy when detecting deregulated miRNAs involved in CaP, which demonstrates its potential utility in profiling and identifying clinical and research biomarkers.
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