Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 18, Pages 7593-7598Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac301429n
Keywords
-
Categories
Funding
- Japan Society for the Promotion of Science (JSPS) [22245011, 23760745]
- Asahi Glass Foundation
- Cabinet Office, Government of Japan
- Grants-in-Aid for Scientific Research [24710140, 22245011, 23760745] Funding Source: KAKEN
Ask authors/readers for more resources
We propose a novel electrochemical detection system for alkaline phosphatase (ALP) activity using the difference in water and oil solubilities between the substrate, ferrocene ethyl phosphate ester (FcEtOPO(3)(2-)), and the enzymatic product, ferroceneethanol (FcEtOH). In this system, water droplets containing ALP and FcEtOPO(3)(2-) were placed on a Pt disk microelectrode and surrounded by a mineral oil. By the ALP-catalyzed reaction, FcEtOPO(3)(2-) was converted to FcEtOH, which was then transferred to the mineral oil from the water droplets with FcEtOPO(3)(2-) remaining in the water droplets. After partitioning FcEtOH from the water droplets, FcEtOPO(3)(2-) was detected at the Pt disk microelectrode to estimate the ALP activity. Using this novel system, the ALP activity of embryoid bodies was successfully detected. We believe that the present system will be widely applicable to ALP-based bioassays.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available