Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 10, Pages 4587-4593Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac300721u
Keywords
-
Categories
Funding
- China Postdoctoral Science Foundation [11175012]
- Chinese Central Universities Funds [FRF-TP-12-171A, 06108037, 06199019, 06108101]
- National Natural Science Foundation of China [21127007, 21075055, 21135002, 21121091]
- National Research Program of China [2010CB732400]
Ask authors/readers for more resources
A simple, highly sensitive, and selective multiple microRNA (miRNA) detection method based on the graphene oxide (GO) fluorescence quenching and isothermal strand-displacement polymerase reaction (ISDPR) was proposed. The capability to discriminate ssDNA and double-stranded nucleic acid structure coupled with the extraordinary fluorescence quenching of GO on multiple organic dye allows the proposed strategy to simultaneously and selectively detect several miRNA labeled with different dyes in the same solution, while the ISDPR amplification endows the detection method with high sensitivity. The strong interaction between ssDNA and GO led to the fluorescent ssDNA probe exhibiting minimal background fluorescence. Upon the recognition of specific target miRNA, an ISDPR was triggered to produce numerous massive specific DNA-miRNA duplex helixes, and a strong emission was observed due to the weak interaction between the DNA-miRNA duplex helix and GO. A miRNA biosensor down to 2.1 fM with a linear range of 4 orders of magnitude was obtained. Furthermore, the large planar surface of GO allows simultaneous quenching of several DNA probes with different dyes and produces a multiple biosensing platform with high sensitivity and selectivity, which has promising application in profiling the pattern of miRNA expression and biomedical research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available