Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 70, Issue 1, Pages 112-118Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2007.04.001
Keywords
biosensor; magnetoelastic; polyclonal antibodies; Salmonella typhimurium; specificity
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In this article, we report the results of an investigation into the performance of a wireless, magnetoelastic biosensor designed to selectively detect Salmonella typhimurium in a mixed microbial population. The Langmuir-Blodgett (LB) monolayer technique was employed for antibody (specific to Salmonella sp.) immobilization on rectangular shaped strip magnetoelastic sensors (2 x 0.4 x 0.015 mm). Bacterial binding to the antibody on the sensor surface changes the resonance parameters, and these changes were quantified as a shift in the sensor's resonance frequency. Response of the sensors to increasing concentrations (5 x 10(1) to 5 x 10(8) cfu/ml) of S. typhimurium in a mixture of extraneous foodborne pathogens (Escherichia coli O157 :H7 and Listeria monocytogenes) was studied. A detection limit of 5 x 103 cfu/ml and a sensitivity of 139 Hz/decade were observed for the 2 x 0.4 x 0.015 mm sensors. Binding kinetics studies have shown that the dissociation constant (Kd) and the binding valencies for water samples spiked with S. typhimurium was 435 cfu/ml and 2.33 respectively. The presence of extraneous microorganisms in the mixture did not produce an appreciable change in the biosensor's dose response behavior. (C) 2007 Elsevier B.V All rights reserved.
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