Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 9, Pages 4199-4206Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac3005633
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Funding
- National Cancer Institute of NIH [K25CA149080]
- University of Florida
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Circulating tumor cells (CTC) in the peripheral blood could provide important information for diagnosis of cancer metastasis and monitoring treatment progress. However, CTC are extremely rare in the bloodstream, making their detection and characterization technically challenging. We report here the development of an aptamer-mediated, micropillar-based microfluidic device that is able to efficiently isolate tumor cells from unprocessed whole blood. High affinity aptamers were used as an alternative to antibodies for cancer cell isolation. The microscope-slide-sized device consists of >59 000 micropillars, which enhanced the probability of the interactions between aptamers and target cancer cells. The device geometry and the flow rate were investigated and optimized by studying their effects on the isolation of target leukemia cells from a cell mixture. The device yielded a capture efficiency of similar to 95% with purity of similar to 81% at the optimum flow rate of 600 nL/s. Further, we exploited the device for isolating colorectal tumor cells from unprocessed whole blood; as few as 10 tumor cells were captured from 1 mL of whole blood. We also addressed the question of low throughput of a typical microfluidic device by processing 1 mL of blood within 28 mm. In addition, we found that, similar to 93% of the captured cells were viable, making them suitable for subsequent molecular and cellular studies.
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