Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 15, Pages 6747-6752Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac301248h
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Funding
- NIH [GM054585-15]
- NSF [CHE-0911330]
- Agilent Foundation
- ANPCyT-UNLP (Argentina)
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0911516] Funding Source: National Science Foundation
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Background correction is a very important step that must be performed before peak detection or any quantification procedure. When successful, this step greatly simplifies such procedures and enhances the accuracy of quantification. In the past, much effort has been invested to correct drifting baseline in one-dimensional chromatography. In fast online comprehensive two-dimensional liquid chromatography (LCxLC) coupled with a diode array detector (DAD), the change in the refractive index (RI) of the mobile phase in very fast gradients causes extremely serious baseline disturbances. The method reported here is based on the use of various existing baseline correction methods of one-dimensional (1D) liquid chromatography to correct the two-dimensional (2D) background in LCxLC. When such methods are applied orthogonally to the second dimension (D-2), background correction is dramatically improved. The method gives an almost zero mean background level and it provides better background correction than does simple subtraction of a blank. Indeed, the method proposed does not require running a blank sample.
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