Journal
JOURNAL OF VIROLOGY
Volume 81, Issue 13, Pages 7310-7315Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00034-07
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Funding
- NIAID NIH HHS [R44 AI056409, 5R44AI056409-06] Funding Source: Medline
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The Tyr-X-X-Leu (YxxL) motif of the vaccinia virus F13L protein was examined for late (L) domain activity. The ability of an F13L deletion virus to form plaques was restored by PCR products containing single alanine substitutions within the motif and a YAAL construct but not by constructs lacking both the Y and L residues. Recombinant viruses possessing alanine substitutions in place of the tyrosine or the leucine residue in the YxxL motif demonstrated small, asymmetrical plaques. RNA interference-dependent depletion of Alix and TSG101 (host proteins involved in L domain-dependent protein trafficking) diminished extracellular enveloped virion production to various degrees, suggesting that the YxxL motif is a genuine L domain.
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