Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 854, Issue 1-2, Pages 43-47Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2007.03.039
Keywords
gabapentin; epilepsy; 1-fluoro-2,4-dinitrobenzene; HPLC; UV-detection
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A rapid, sensitive and accurate high -performance liquid chromatographic method with UV detection was developed and validated for the quantification of gabapentin in human plasma. Gabapentin was quantified using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene following protein precipitation of plasma with acetonitrile. Amlodipine was used as internal standard. The chromatographic separation was carried out on a Nova-Pak Cis column using a mixture of 50 mM NaH2PO4 (pH = 2.5)-acetonitrile (30:70, v/v) as mobile phase with UV detection at 360 nm. The flow rate was set at 1.5 ml/min. The method was linear over the range of 0.05-5 mu g/ml of gabapentin in plasma (r(2) > 0.999). The within-day and between-day precision values were in the range of 2-5%. The limit of quantification of the method was 0.05 mu g/ml. The method was successfully used to study the pharmacokinetics of gabapentin in healthy volunteers. (C) 2007 Elsevier B.V. All rights reserved.
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