1H-MRS of the macaque monkey primary visual cortex at 7 T:: strategies and pitfalls of shimming at the brain surface

Magnetic resonance spectroscopy (MRS) is ideally suited for physiology-neurochemistry experiments with the living brain and particularly for studies on the primary visual cortex (striate cortex or area VI). Yet, the highly convoluted form of the human VI has thus far prevented the performance of MRS investigations that are spatially confined within the gray matter of this area. Typically, these studies are compromised by partial volume contaminations originating from white matter tissue, cerebrospinal fluid and other cortical areas. In this study, was exploited the relative flatness of VI in macaques to enable single-voxel H-1-MRS from a small volume (5 x 1.6 x 5 mm(3), 40 mu l) that was entirely confined within the VI gray matter of anesthetized monkeys. Linewidths of 13.5 +/- 1.9 Hz and 13.0 +/- 1.3 Hz for water and creatine, respectively, were achieved with a two-step shimming strategy for voxels at the brain surface. The quality of the obtained results paves the way for further neuroscientific research, including studies on the cortical microcircuits and the dynamic longitudinal changes occurring during cortical reorganization and plasticity. (c) 2007 Elsevier Inc. All rights reserved.