Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 3, Pages 850-855Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac102445r
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Funding
- Swiss Initiative in Systems Biology (SystemsX.ch)
- ETH Zurich
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In this article, we introduce a method using nanoscale ion-pair reversed-phase high-performance liquid chromatography (nano-IP-RP-HPLC) hyphenated to nanoelectrospray ionization high-resolution mass spectrometry (nano-ESI-HRMS) to separate and identify metabolites in cell extracts. Separation of metabolites was performed on a 100 mu m i.d. C18 column with tributylamine (TBA) as the ion-pairing reagent and methanol as the eluent. Basic pH (9.4) of the mobile phase was critical to achieve sufficient retention and sharp metabolite elution at a low concentration of TBA (1.7 mM). Limits of detection were determined for standards with an LTQ-Orbitrap mass spectrometer to be in the upper attomole to low femtomole range for key metabolites such as nucleotides, phosphorylated sugars, organic acids, and coenzyme A thioesters in solvent as well as in a complex matrix. To further evaluate the method, metabolome analysis was performed injecting different amounts of biomass of the methylotroph model organism Methylobacterium extorquens AM1. A C-12/C-13 labeling strategy was implemented to improve metabolite identification. Analysis of three biological replicates performed with 1.S ng of cell dry weight biomass equivalents resulted in the identification of 20 +/- 4 metabolites, and analysis of 150 ng allowed identifying 157 +/- 5 metabolites from a large spectrum of metabolite classes.
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