Journal
ACTA BIOMATERIALIA
Volume 3, Issue 4, Pages 475-484Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2007.02.001
Keywords
polycaprolatone; tissue engineering; polyurethane; osteoblast; modulus
Funding
- NIAMS NIH HHS [R21 AR051945-02, R21 AR051945, R21 AR015945] Funding Source: Medline
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Segmented polyurethanes have been used extensively in implantable medical devices, but their tunable mechanical properties make them attractive for examining the effect of biomaterial modulus on engineered musculoskeletal tissue development. In this study, a family of segmented degradable poly(esterurethane urea)s (PEUURs) were synthesized from 1,4-diisocyanatobutane, a poly(F-caprolactone) (PCL) macrodiol soft segment and a tyramine-1,4-diisocyanatobutane-tyramine chain extender. By systematically increasing the PCL macrodiol molecular weight from I 100 to 2700 Da, the storage modulus, crystallinity and melting point of the PCL segment were systematically varied. In particular, the melting temperature, T-m, increased from 21 to 61 degrees C and the storage modulus at 37 degrees C increased from 52 to 278 MPa with increasing PCL macrodiol molecular weight, suggesting that the crystallinity of the PCL macrodiol contributed significantly to the mechanical properties of the polymers. Bone marrow stromal cells were cultured on rigid polymer films under osteogenic conditions for up to 21 days. Cell density, alkaline phosphatase activity, and osteopontin and osteocalcin expression were similar among PEUURs and comparable to poly(D,L-lactic-coglycolic acid). This study demonstrates the suitability of this family of PEUURs for tissue engineering applications, and establishes a foundation for determining the effect of biomaterial modulus on bone tissue development. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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