Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 14, Pages 5754-5761Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac201123t
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Funding
- Austrian Science Foundation [L472]
- National Science Foundation [CHE NSF 0848650, NSF 0852740-DBI]
- Austrian Science Fund (FWF) [L 472] Funding Source: researchfish
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0848650] Funding Source: National Science Foundation
- Austrian Science Fund (FWF) [L472] Funding Source: Austrian Science Fund (FWF)
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The ambient mass spectrometry technique, desorption electrospray ionization mass spectrometry (DESI-MS), is applied for the rapid identification and spatially resolved relative quantification of chlorophyll degradation products in complex senescent plant tissue matrixes. Polyfunctionalized nonfluorescent chlorophyll catabolites (NCCs), the final products of the chlorophyll degradation pathway, are detected directly from leaf tissues within seconds and structurally characterized by tandem mass spectrometry (MS/MS) and reactive-DESI experiments performed in situ. The sensitivity of DESI-MS analysis of these compounds from degreening leaves is enhanced by the introduction of an imprinting technique. Porous polytetrafluoroethylene (PTFE) is used as a substrate for imprinting the leaves, resulting in increased signal intensities compared with those obtained from direct leaf tissue analysis. This imprinting technique is used further to perform two-dimensional (2D) imaging mass spectrometry by DESI, producing well-resolved images of the spatial distribution of NCCs in senescent leaf tissues.
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