Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 15, Pages 5949-5956Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac201340s
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Funding
- National Institutes of Health (NIH), part of the NIH Roadmap for Medical Research [1-DP2-OD002190-01]
- Center for Advanced Study at the University of Illinois at Urbana-Champaign
- Camille and Henry Dreyfus Foundation
- Eastman Chemical Company
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In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and a silicon photonic microring resonator array transduction platform. Microring resonator arrays are covalently finictionalized with DNA capture probes that are complementary to solution phase miRNA targets. Following hybridization on the sensor, the anti-DNA:RNA antibody is introduced and binds selectively to the heteroduplexes, giving a larger signal than the original miRNA hybridization due to the increased mass of the antibody, as compared to the 22-mer oligoribonucleotide. Furthermore, the secondary recognition step is performed in neat buffer solution and at relatively higher antibody concentrations, facilitating the detection of miRNAs of interest. The intrinsic sensitivity of the microring resonator platform coupled with the amplification provided by the anti-DNA:RNA antibodies allows for the detection of microRNAs at concentrations as low as 10 pM (350 amol). The simplicity and sequence generality of this amplification method position it as a promising tool for high-throughput, multiplexed miRNA analysis as well as a range of other RNA based detection applications.
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