Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 3, Pages 648-653Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac103047c
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Funding
- National Natural Science Foundation of China [41076047]
- Chinese Academy of Sciences [KZCX2-YW-205-03]
- Shandong Provincial Natural Science Foundation, China [ZR2010DM004]
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Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
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