Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 18, Pages 6918-6923Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac201640d
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Funding
- USDoE Office of Biological and Environmental Research
- NIH National Center for Research Resources [RR18522]
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Protein identification in bottom-up proteomics requires disentangling isomers of proteolytic peptides, a major class of which are sequence inversions. Their separation using ion mobility spectrometry (IMS) has been limited to isomeric pairs. Here we demonstrate baseline separation of all seven 8-mer tryptic peptide isomers using differential IMS. Evaluation of peak capacity implies that even larger libraries should be resolved for heavier peptides with higher charge states.
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