Journal
REPRODUCTIVE SCIENCES
Volume 14, Issue 5, Pages 445-455Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1933719107306226
Keywords
GLUT8; GLUT9a; GLUT9b; testis; sperm
Categories
Funding
- NICHD NIH HHS [R01 HD040390] Funding Source: Medline
- NIDDK NIH HHS [P30 DK056341-07, P30 DK056341-06, P30 DK056341] Funding Source: Medline
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The objective of this study is to confirm the precise glucose transporter (GLUT) 8 localization and determine the expression of GLUT9a and GLUT9b by Western blot and confocal and immunoelectron microscopy in the mouse testis and sperm. GLUT8, GLUT9a, and GLUT9b proteins are expressed in the most intraseminiferous tubula cells and Leydig cells. GLUT8 localizes in the mid-piece and principal piece as well as in the acrosomal region of the sperm. Immunoelectron microscopic analysis shows that GLUT8 is strongly detectable at the acrosome and neck region of the sperm, In the midpiece, GLUT8 localizes at the outer dense fibers (odf) as well as at the circumference of the spiral mitochondria. In the principal piece, GLUT8 localizes at the odf. GLUT9a strictly localizes in the midpiece, but GLUT9b localizes in the acrosome, midpiece, and principal piece of the sperm, These results suggest that glucose uptake via GLUT8, GLUT9a, and GLUT9b likely affects normal spermatogenesis, steroidogenesis, and sperm function in the mouse.
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