Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 23, Pages 8906-8912Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac201298r
Keywords
-
Categories
Funding
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology (MEST) of the Korean government [313-2008-2-C00538, 2008-0062074, 2011-0017356]
- NRF
- MEST [2008-2004457]
- National Research Foundation of Korea [2008-2004457] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
