Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 20, Pages 7603-7607Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac2018926
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Funding
- National Natural Science Foundation of China [20975034, 21177036]
- National Key Scientific Program of China [2011CB-911001, 2011CB911003]
- Hunan Provincial Natural Science Foundation of China [11JJ1002]
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Fluorescence catalytic beacons have emerged as a general platform for sensing applications. However, almost all such sensing systems need covalent modification of the DNAzymes with fluorophore-quencher pairs, which may require elaborate design of the synthetic routes and many heavy and complicated synthetic steps and result in increased cost and lower synthesis yield. Here we report the construction of fluorescent cascadic catalytic beacons. With separation of the molecular recognition module from the signal reporter, this new design both avoids DNAzyme modifications and improves sensitivity through an endonuclease-based cascadic enzymatic signal amplification. This allows detection of L-histidine with high sensitivity (LOD = 200 nM) and excellent specificity. The proposed sensing system has also been used for detection of L-histidine in cellular homogenate with satisfactory results.
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