Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 11, Pages 4110-4117Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac200253x
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Funding
- NSF [ECCS 0901292]
- NIH/NIAID [U01 AI 082457]
- DARPA [N66001-10-1-4003, N660001-09-C-2082]
- Natural Science and Engineering Research Council, Canada
- Technion
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [0901292, 0900899] Funding Source: National Science Foundation
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We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria specific sequences. We demonstrated detection of E. coli in bacteria cultures as well as in patient urine samples in the clinically relevant range 1E6-1E8 cfu/mL. For bacterial cultures we further demonstrate quantification in this range. The assay requires minimal sample preparation (a single centrifugation and dilution), and can be completed, from beginning of lysing to detection, in under 15 min. We believe that the principles presented here can be used for design of other rapid diagnostics or detection methods for pathogenic diseases.
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