4.7 Article

Abortive Infection of Snakehead Fish Vesiculovirus in ZF4 Cells Was Associated with the RLRs Pathway Activation by Viral Replicative Intermediates

Journal

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 16, Issue 3, Pages 6235-6250

Publisher

MDPI
DOI: 10.3390/ijms16036235

Keywords

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Funding

  1. Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [52204-12020]
  2. Fundamental Research Funds for the Central Universities [2013PY069, 2014PY035]
  3. Wuhan Chopper Fishery Co., Ltd. [720107-137076]
  4. Natural Science Foundation of China [31372563, 31402341]
  5. Postdoctoral Science Foundation [2014M550400]
  6. State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2015FB05]

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Snakehead fish vesiculovirus (SHVV) is a negative strand RNA virus which can cause great economic losses in fish culture. To facilitate the study of SHVV-host interactions, the susceptibility of zebrafish embryonic fibroblast cell line (ZF4) to the SHVV was investigated in this report. The results showed that high amount of viral mRNAs and cRNAs were detected at the 3 h post-infection. However, the expressions of the viral mRNAs and cRNA were decreased dramatically after 6 h post-infection. In addition, the expressions of interferon (IFN) and interferon-induced GTP-binding protein Mx were all up regulated significantly at the late stage of the infection. Meanwhile, the expressions of Retinoic acid-inducible gene I (RIG-I) and Melanoma differentiation-associated gene 5 (MDA5) were also all up-regulated significantly during the infection. Two isoforms of DrLGP2 from zebrafish were also cloned and analyzed. Interestingly, the expression of DrLGP2a but not DrLGP2b was significantly up-regulated at both mRNA and protein levels, indicating that the two DrLGP2 isoforms might play different roles during the SHVV infection. Transfection experiment showed that viral replicative intermediates were required for the activation of IFN-alpha expression. Taken together, the abortive infection of SHVV in ZF4 cells was associated with the activation of RLRs pathway, which was activated by viral replicative intermediates.

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