Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 4, Pages 1456-1461Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac103155f
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Funding
- DIA-NET, the Graduate School of Advanced Diagnostic Technologies and Applications
- Tekes, the Finnish Funding Agency for Technology and Innovation
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We have developed a robust array-in-well test platform based on an oligonudeotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.
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