Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 6, Pages 2250-2257Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac103079q
Keywords
-
Categories
Funding
- National Institutes of Health (NIH) [R33CA099139-01, 1S10RR023044-01, 1U54AI57141-01]
- NIH/NIGMS Center for Systems Biology [P50 GM076547]
- Swiss National Science Foundation (SNF) [PBLAA-119623]
- University of Washington's Proteomics Resource [UWPR95794]
Ask authors/readers for more resources
Data-dependent precursor ion selection is widely used in shotgun proteomics to profile the protein components of complex samples. Although very popular, this bottom-up method presents major drawbacks in terms of detectable dynamic range. Recently, we demonstrated the superior performance of a data-independent method we termed precursor acquisition independent from ion count (PAcIFIC). Here, we report a faster, accurate, multiplexed, and quantitative PAcIFIC method. Our results show that the time needed to perform such analysis can be decreased by 33% to 66% using modern ion trap instruments and that high mass accuracy can be applied to such a strategy. Quantification capability is demonstrated on protein standards and a whole bacterial cell lysate using isobaric tagging. Finally, we confirm in yeast the dynamic range capabilities of such a method where proteins down to less than 50 copies per cell can be monitored without sample prefractionation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available