Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 75, Issue 6, Pages 1225-1232Publisher
SPRINGER
DOI: 10.1007/s00253-007-0964-2
Keywords
protein engineering; directed evolution; enzymes
Categories
Ask authors/readers for more resources
The extraordinary properties of natural proteins demonstrate that life-like protein engineering is both achievable and valuable. Rapid progress and impressive results have been made towards this goal using rational design and random techniques or a combination of both. However, we still do not have a general theory on how to specify a structure that is suited to a target function nor can we specify a sequence that folds to a target structure. There is also overreliance on the Darwinian blind search to obtain practical results. In the long run, random methods cannot replace insight in constructing life-like proteins. For the near future, however, in enzyme development, we need to rely on a combination of both.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available