Journal
ANALYTICAL CHEMISTRY
Volume 83, Issue 21, Pages 8267-8272Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac202077x
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Funding
- National Science Council
- MOE-ATU of Taiwan
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Endogenous hypochlorous acid (Hod) secreted by leukocytes plays a critical role in both the immune defense of mammalians and the pathogenesis of-various diseases intimately related to inflammation. We report the first selective and absolute quantification of endogenous HOCl produced by leukocytes in vitro and in vivo with a novel quantum dot-based sensor. An activated human neutrophil secreted 6.5 +/- 0.9 x 10(8) HOCl molecules into its phagosome, and kinetic measurement for the secretions showed that the extracellular generation of HOCl was temporally retarded, but the quantity eventually attained a level comparable with its intraphagosomal counterpart with a delay of about 1.5 h. The quantity of HOCl secreted from the hepatic leukocytes of rats with or without stimulation of lipopolysaccharide was also determined. These results indicate a possibility to extend our approach to not only clinical settings for quantitative assessment of the bactericidal capability of isolated leukocytes of patients but also fundamental biomedical research that requires critical evaluation of the inflammatory response of animals.
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