Journal
GASTROENTEROLOGY
Volume 133, Issue 1, Pages 278-287Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1053/j.gastro.2007.04.032
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- NIDDK NIH HHS [KO8 #DK60710-06, U01 DK062453, K08 DK060710-06, UO1 #DK062453, K08 DK060710] Funding Source: Medline
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Background & Aims: Biliary atresia is an inflammatory, fibrosclerosing neonatal cholangiopathy, characterized by a periductal infiltrate composed of CD4(+) and CD8(+) T cells. The pathogenesis of this disease has been proposed to involve a virus-induced, subsequent autoreactive T cell-mediated bile duct injury. Antigen-specific T-cell immunity involves clonal expansion of T cells expressing similar T-cell receptor (TCR) variable regions of the beta-chain (V beta). We hypothesized that the T cells in biliary atresia tissue expressed related TCRs, suggesting that the expansion was in direct response to antigenic stimulation. Methods: The TCR V beta repertoire of T cells from the liver, extrahepatic bile duct remnants, and peripheral blood of biliary atresia and other cholestatic disease controls were characterized by fluorescent-activated cell sorter analysis, and TCR junctional region nucleotide sequencing was performed on expanded TCR V beta regions to confirm oligoclonality. Results: FACS analysis revealed V beta subset expansions of CD4(+) and CD8(+) T cells from the liver or bile duct remnant in all patients with biliary atresia and only 1 control. The CD4(+) TCR expansions were limited to V beta 3, -5, -9, and -12 T-cell subsets and the CD8+ TCR V beta expansions were predominantly V beta 20. Each V beta subset expansion was composed of oligoclonal populations of T cells. Conclusions: Biliary atresia is associated with oligoclonal expansions of CD4(+) and CD8(+) T cells within liver and extrahepatic bile duct remnant tissues, indicating the presence of activated T cells reacting to specific antigenic stimulation. Future studies entail identifying the specific antigen(s) responsible for T-cell activation and bile duct injury.
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