Journal
ANALYTICAL CHEMISTRY
Volume 84, Issue 1, Pages 465-469Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac202438a
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Funding
- Chinese Academy of Sciences
- National Natural Sciences Foundation of China [20927006, 21175139, 10804088]
- Academia Sinica, Taiwan
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Due to its strong ultraviolet absorption, high salt tolerance, and little interference in the low molecular weight region, N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) has been applied as a matrix to measure the level of glucose in rat brain microdialysates by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in combination with in vivo microdialysis. By monitoring the ion signals of (glucose + C1)(-) in the mass spectra, we achieved a low detection limit of similar to 10 mu M for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification. It is concluded that NEDC-assisted laser desorption/ionization (LDI) MS is a fast and general method for sensitive detection of small molecules (such as glucose and amino adds) in high ionic strength solutions.
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