Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 14, Pages 6148-6153Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac100940v
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Funding
- National Natural Science Foundation of China [20975055]
- National Natural Science Foundation of Tianjin [08JCZDJC21200]
- National Basic Research Program of China [2006CB705700]
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Some G-quadruplex-hemin complexes can be used as peroxidase-mimicking DNAzymes, catalyzing H2O2-mediated reactions such as the oxidation of 2,2'-azinobis (3-ethylbenzothiozoline)-6-sulfonic acid (AIM) by H2O2. However, some challenges, for example, the relatively low catalytic activity and the disproportionation of the reaction product ABTS.(+), may seriously restrict further development and applications of these complexes. Here, we demonstrated the positive effect of adenosine triphosphate (ATP) on G-quadruplex-hemin DNAzyme-mediated catalytic reactions. The presence of ATP not only improved the catalytic activity of G-quadruplex-hemin DNAzymes, but also inhibited the disproportionation of ABTS.+. These observations may improve the performance of existing G-quadruplex-hemin DNAzyme-based chemical sensors, for example, the Ag+-detection method that uses G-quadruplex-hemin DNAzymes, and widen the application range of G-quadruplex-hemin DNAzymes. We also demonstrated that the phosphate groups, nucleobase, and sugar of ATP determine the reaction-promoting ability of ATP. These observations may be helpful in the design of highly efficient enhancers for G-quadruplex-hemin DNAzymes.
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