Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 7, Pages 3094-3098Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac100357u
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- ONR
- NIH
- ARO Institute for Collaborative Biotechnologies (ICB)
- Armed Forces Institute for Regenerative Medicine (AFIRM)
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB009764, R21EB009518] Funding Source: NIH RePORTER
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We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle-dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(I) phase synchrony (similar to 84%) and throughput (3 x 10(6) cells/h per microchannel).
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