Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 4, Pages 1213-1220Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac9029778
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Funding
- Shorai Foundation for Science and Technology
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A novel fluorimetric method for determining radicals using the natural phenol sesamol as a fluorogenic reagent is reported. In this assay, sesamol was reacted with aqueous radicals to yield one isomer of a sesamol dimer exclusively. The dimer emitted purple fluorescence near 400 nm around neutral pH, where it assumed the monoanionic form. This method was applied to the straightforward detection of radical nitric oxide (NO). The ready availability of sesamol should enable rapid implementation of applications utilizing this new assay, particularly in high-throughput analysis or screening.
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